Measuring total body water with radiolabelled tritium. How to measure the fluid volume intra and extra cellular total body water constitutes in men about 60 of body weight in women 50 is divided into icf and ecf. Ecf is further divided into interstitial extravascular and bloodplasma intravascular compartments. A marker is injected that will be evenly distributed in all the compartments of body fluids. Body fluids can be discussed in terms of their specific fluid compartment a location that is largely separate from another compartment by some form of a physical barrier. Marker that diffuses freely not only in the water outside the cells but also crosses the cell membrane to reach the intracellular fluid.
Rapid mixing of tritiated water throughout all compartments occurs during a 3 to 4 hour equilibration period. Measurement of body fluid volumes total body water is measured using dilution principle. 222 total body water. If one were that way inclined one could also go on to read this indepth article by shoeller etal from 1980 full of thick juicy physiology. Fluid intake and output daily intake of water 1. Liquids or water in the food 2100 mlday 2.
This is estimated by measuring the volume of distribution of isotopes of water. Synthesis in the body as a result of oxidation of carbohydrates adding about 200 mlday daily loss of body water 1. Fluid loss in sweat 100 mlday 3. Insensible water loss 700 mlday 2. Composition of fluid intra and extracellular. The intracellular fluid icf compartment is the system that includes all fluid enclosed in cells by their plasma membranes.
Tritium oxide tho is used because it is a weak beta emitter making it easy to measure in a liquid scintillation counter. 18112012 14 copyright 2004 pearson education inc publishing as benjamin cummings. Sinan canan created date. Water loss in feces 100 mlday 4. Body fluid compartments thursday december 10 2015 6. Not prone to changing the distribution of fluid between compartments.
So simple one can describe it using only pictures. Distribution of total bodyy water compartments volume l percentage body weight body water total body water 42 60 100 icf 28 40 67 ecf 14 20 33 plasma 25 35 5 8 interstitial fluid transcellular fluid mesenchymal tissue 75 105 15 25 thursday december 10 2015 7.